African swine fever is caused by the African swine fever virus. ASFV infects domestic pigs, warthogs, and South African wild boars. It spreads through direct contact with non animals, indirect contact with contaminated feed, and through biological carriers (soft ticks). ASF can occur in various forms including acute, sub acute, and sub acute.
What methods do you know for detecting African swine fever?
African swine fever is similar and complex to traditional swine fever, clinically resembling acute swine fever, characterized by high fever, skin congestion, miscarriage, and organ bleeding. Due to the fact that China did not belong to the African swine fever epidemic before, there was no relevant targeted research. Currently, the detection method used is the one jointly developed by the former Ministry of Agriculture Quarantine Institute and the Meidao Animal Disease Research Institute in the United States in 1997, mainly PCR and ELISA, following the diagnostic skills of GB/T18648-2002 African swine fever.
With the development of relevant detection skills, the detection methods for African swine fever are also relatively complete. PCR is currently a simple molecular detection method, but its sensitivity is limited, so new methods based on PCR have emerged.
1. The fluorescence quantitative PCR method introduces spectroscopy into the PCR reaction and quantitatively determines the amount of specific amplification products through changes in the strength of fluorescence signals, solving the problems of low sensitivity of conventional PCR methods and environmental pollution caused by ethidium bromide in electrophoresis detection.
2. Isothermal amplification method is an emerging molecular biology detection method that does not require denaturation or annealing steps in PCR, and can complete the amplification of target sequences, greatly reducing the time. The entire amplification reaction time is generally less than 60 minutes, while the reaction time of conventional PCR methods is generally more than 2 hours. The isothermal amplification kit is suitable for on-site detection, with high sensitivity, and can significantly increase the feasibility of preventing and controlling swine fever.
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